Peggy Woolley, formerly Archer, was often viewed as a traditionalist, conservative character in the soap opera charting the ups and downs of life in fictional village of Ambridge.
Paying tribute, Radio 4 controller Mohit Bakaya said: "June Spencer has been a longstanding presence and companion for Radio 4 listeners during her exceptional run on The Archers.
"Many have grown up with June as Peggy and listened as she journeyed through life’s many chapters, with all of its ups and downs. In her later years, her portrayal of a devoted wife caring for a husband with dementia, including their very moving final goodbye, was deeply poignant and powerful radio.
"We send all our love and condolences to June’s family and the many people whose lives she touched."