According to Garcia, the committee has also received records from JP Morgan and Deutsche Bank, which they intend to release "in the days ahead".
Little St James was one of two islands in the Virgin Islands owned by Epstein.
In 2022, the attorney general of the US territory reached a settlement of over $105m (£78.6m) after local authorities alleged that "dozens of young women and children" were trafficked, raped and assaulted on the two islands.
The images shed little new light on the case, besides providing a glimpse of one of Epstein's crime scenes and opulent lifestyle in the Virgin Islands.
The release, however, comes as the Trump administration remains under pressure to release the broader set of documents kept by the US justice department, with Garcia saying in his statement that "it's time for President Trump to release all the files, now".
Later on Wednesday, the committee released a second batch of some 200 images as well as several videos. Most of those showed the same rooms as the first batch, with some new close-ups of the apparent dentist chair and the masks on the walls of that room.
All of the masks are in a similar style and depict male faces.
Other images show personal items around the house, such as a large selection of shampoos and conditioners and various art items including statues and paintings.
There is also a picture of Epstein and Maxwell meeting Pope John Paul II.
The photos seem to have been taken in 2020 according to the metadata - after the death of Jeffrey Epstein in 2019 - so the house appears to have been packed away, with furniture stacked up and artwork removed from walls.