"Without being able to make noise, we can’t make money," says Edie Kench-Andrews, general manager of the Moth Club, in Hackney.
She is worried that the independent music and comedy venue will be shut down by noise complaints if a block of flats is built next door.
The venue, which opened in 1972 as a servicemen's club, has hosted a range of big name artists including Lady Gaga, Dave Grohl and Rick Astley since 2015.
Hackney council has said the application is being considered. Those behind the plans have refused to comment.