During his performance on Saturday, Glover, 42, sat down on the stage as slow music continued in the background, videos widely shared on social media show.
Glover - who is famous for both his music and his acting roles in shows like Community, Atlanta, and Mr and Mrs Smith - joked that he was copying fellow US actor and comedian Jamie Foxx, who suffered a stroke in 2023 that also at first presented as a bad headache.
On stage, Glover then turned more serious, telling the crowd that when he first found out he had had a stroke, he felt like he was letting everyone down. Specifically, he said, he had always promised to perform in Ireland, but he still hasn't been able to.
Glover added that doctors also found a hole in his heart, so he had to have two surgeries.
"They say everybody has two lives and the second life starts when you realise you have one," Glover said.
"You got one life, guys, and I gotta be honest, the life I've lived with you guys has been such a blessing," he told fans.
When Glover first postponed his world tour in September 2024, he wrote on social media that he had to focus on his "physical health for a few weeks", but still urged his fans to hang onto their tickets. But a month later, Glover announced he was forced to cancel the rest of the tour because of an "ailment" that required tests, surgery, and "time out to heal".
"As my wife says, which I love, 'If this life is the flavour, as soon as I'm done, give me another scoop'", Glover told the crowd on Saturday after revealing his medical struggles.