Sir Keir Starmer has said he has "been assured" that anonymous briefings against cabinet ministers earlier this week "did not come from Downing Street".
In a bid to draw a line under the row, he said he had "made it clear" to his team that such activities were "unacceptable".
On Wednesday, Sir Keir apologised to Wes Streeting, after his allies privately told news outlets the health secretary could be planning to make a leadership challenge.
Many Labour MPs remain sceptical about the claim the briefings did not come from No 10 and blame the prime minister's chief of staff Morgan McSweeney, not necessarily for the briefing, but for overseeing the strategy and culture of Downing Street.