The Agriculture Department unveiled what it called a "big package" this week aimed at ramping up domestic beef production by opening more land for cattle grazing and supporting small meat processors.
That proposal came after Trump drew the ire of ranchers when he proposed importing more beef from Argentina, potentially quadrupling US purchases.
Eight House Republicans responded with a letter expressing concerns about the plans.
Even the National Cattlemen's Beef Association, which has voiced support for Trump's policies in the past, said the import plan "only creates chaos at a critical time of the year for American cattle producers, while doing nothing to lower grocery store prices".
Trump responded by assuring farmers that he was helping them in other ways, noting that tariffs that were limiting imports from Brazil.
"It would be nice if they would understand that, but they also have to get their prices down, because the consumer is a very big factor in my thinking, also," Trump wrote.
But that has failed to quell the furore.
Justin Tupper, president of the US Cattlemen's Association, says only the big four meat packers would benefit from Trump's import plan in his view.
"I don't see that lowering prices here at all," he says.