Doechii drew a huge crowd to the West Holts Stage - including pop star Harry Styles, who danced away to her brief set in the middle of the field, unbothered by fans.
The Florida-born rapper, known to fans as the Swamp Princess, sang, rapped, danced and changed outfits multiple times, in a show that was themed as a lesson in the history of hip-hop.
Her flow was flawless on breakout tracks like Persuasive, Anxiety, Denial Is A River and Alter Ego - and she even made fun of herself, recreating a popular social media clip from this year's Met Gala, where she was overhead demanding "more umbrellas" to cover her body as she changed outfits.
She was aided by her eight dancers, who climbed over props, twerked and even vogued while descending a slide.
Doechii's rise over the past year has been nothing short of phenomenal and this slick, bespoke Glastonbury performance felt like a new feather in her cap.
Scissor Sisters, meanwhile, continued their reunion by packing out the Woodsies tent for a set that reminded everyone how many classics they'd written - from I Don't Feel Like Dancing and Laura to the everso sleazy Filthy/Gorgeous.
Beth Ditto joined them for the latter, while Jessie Ware took a celebratory strut through I Don't Feel Like Dancin', and Sir Ian McKellen recreated his monologue from the 2010 single Invisible Light.
As he stood in the wings, fans started chanting "Oh, Ian McKellen" to the tune of The White Stripes' Seven Nation Army, at which the actor clasped his hands to his face in shock.