England A will host Australia A at Twickenham Stoop on Sunday, a game which follows February's 91-5 victory over Portugal that marked the return of A fixtures after eight years.
Another fixture will follow in February against Ireland A at Bristol's Ashton Gate as the RFU aims to get back to more regular fixtures to help help bridge the gap between Premiership and Test rugby.
After his success with England Under-20s Mark Mapletoft has been named head coach for Sunday's fixture and is joined by forwards coach Andy Titterrell, who was also involved with the summer's World Championship-winning side.
The A squad contains a mixture of under-20 winners including Northampton Saints flanker Henry Pollock and Gloucester prop Afolabi Fasogbon, but also senior internationals such as Tom Willis, Joe Heyes, Curtis Langdon, Tom Pearson and Fraser Dingwall.
"I think it is a great progression and keeps the alignment in-between the pathway and the seniors," Titterrell said.
"It is showing the importance and hard work going on behind the scenes to give those boys the exposure so they can see what that next level feels like."